Here are the keys. Unlock the full potential of your research model using our newest innovation, a peptide-based gene delivery system.
With great excitement we annouce our new product line. BPP has engineered a novel multi-functional designer protein termed BPP Bioportide™. This agent quickly, spontaneously, reversibly, and reliably binds nucleic acids and transports them across the host’s cell membrane utilizing a specialized, N-terminal cell penetrating peptide moiety. Upon membrane crossing, nucleic acid is released to the cytoplasma and becomes functional.
Figure | Mock-up of our new BPP Bioportide™ Kit for bacterial transformation.
From our and our scientific partners' studies, it becomes clear that BPP Bioportide™ are capable of transforming and transfecting a wide range of model organisms from various domains of life, showing particularly interesting results not only in bacteria (e.g. Steptomyces) but also lower and higher eukaryotes (e.g. yeast and plants). For this reason, we have put together a test kit comprising 10 of our most potent BPP Bioportide™ variants for you to transform your model organism of choice. Find which BPP Bioportide™ is working best for you, we are looking forward to supporting you in your research. Learning from one another, we hope to accelerate research and find tailored solutions in gene delivery.
Table | Overview of BPP Bioportide™ available in the kit.
Legend:
PMP:plant membrane penetrator*) * exact target spectrum is subject of research. Stated expected target spectrum is inferred from either sequence homology, prediction, or experimental data.
References:
1) Cardoso et al. (2012) BBA - Biomembranes 1818: 877-8882)
2) Numata et al. (2018) Scientific Reports 8: 109663)
3) Guo et al. (2016) Curr Genomics 17: 476-489
There are no limits towards the nucleoic acid that can be introduced using the BPP Bioportide™ Technology. RNA or DNA, small or large plasmids, or fragments. Your choice.
Using the BPP Bioportide™, large plasmid libraries can be transfered at once or in parallel by robust and straight-forward protocols.
BPP Bioportide™ are capable to traffic nucleoic acid across viable previously untreated cell membranes.
Fitness is an important factor of successful gene delivery. Only cells that survive the treatment are able to carry the genetic material introduced.
The BPP Bioportide™ allows to work with healty and growing organisms which recover faster than, e.g., organisms from cryoculture. Watch your model strains reach higher yields faster.
Some model organisms are just labor- and time consuming to transfect/transform using common methods. Switching to BPP Bioportides™ saves time and money.
"Next Generation Transformation"
As a branch of the Better Peptides Platform Technology, BPP Bioportide™ inherit a few handy functionalities.
First, BPP Bioportide™ contain a signal driving expression to inclusion bodies in bacterial cultures. This way, production of BPP Bioportide™ benefits from all the Better Peptides advantages.
Second, the cargo binding domain has been optimized to recruit nucleic acids (i.e., DNA and RNA) via the ribose carbohydrate residue, a spontanous and reliable complex formation ensures multivalent binding of both, cargo and host cell for successful transformation/transfection of the host cell. And last but not least, a specialized cell penetrating peptide moiety carries the key to the host cell. From a growing collection of cell penetrating peptide sequences, we find those that are most effective in penetrating important model organisms and cell types across all kingdoms of life.
Today, we begin to share our findings on the most basic and probably most important of research model organisms, the gram-negative bacterium Escherichia coli. More will follow in due time.
Figure | BPP Bioportide™ model as predicted by Google Alphafold 3. Molecular model was visualized with UCSF ChimeraX (University of California, San Francisco, curtousy of Brunner Lab, Universität Heidelberg, Germany) and further edited in Adobe Photoshop.
Figure | Graphical Abstract of the BPP Bioportide™ workflow.
Minimum preparation, easy workflow, fast results.
The protocol is remarkably easy and fast. Purified nucleic acids are mixed with a BPP Bioportide™ solution in a prescribed ratio, using our recommended buffer system.
Within minutes, BPP Bioportides™ will recruit nucleic acids spontaneously to form an active complex. Simply add this solution to mid-growth phase bacteria, incubate briefly, and grow the cells on or in selective growth media – done. It is that easy.
Excellent transformation efficiency shown with E.coli B27 cells and a 10 kb plasmid (pUC based). No special competency needed.
BPP Bioportide™ can deliver even large plasmids, while keeping cells vital and ready to grow. Neither incubation period on ice nor heat shock is needed, both preserving cells vitality. Rapid kick-in of growth was demonstrated using in solution online growth monitoring (aquilabiolabs GmbH / sbi, MPS-System).
Figure | Comparison of transformation efficiencies in heat-shock treated Ca2+ competent cells and Bioportide BP17 treated non-competent E.coli DH5alpha cells (invitrogen) using (A) pUG plasmid and (b) a 10 kb plasmid encoding for GFP.
Interested in a test drive? Thinking about a great application? Having troubles transforming a specfic host cell or cell type? - Talk to us for a personalized offer! Our scientists will be happy to advise you tailored to your individual needs.
We have an entire repertoire of BPP Bioportide™ of yet to be characterized target spectrum at hand. Let us collaborate and find the optimal solution for you and others in your field of research having the same troubles!
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